Recently CRISPR/Cas9 platform announces the launch of its off-target testing service for CRISPR/Cas9-mediated gene editing.
Shirley, USA - July 22, 2019 /MarketersMedia/ —
New York, Jul 22th, 2019 - CRISPR/Cas9 platform, which is a professional division platform of Creative Biogene, providing comprehensive gene editing services and products in order to offer the most reliable and professional services and products for customers worldwide. Recently CRISPR/Cas9 platform announces the launch of its off-target testing service for CRISPR/Cas9-mediated gene editing.
CRISPR/Cas9 Platform provides complete off-target analysis, ranging from selection strategies to raw data analysis. The analysis methods include PCR-based analysis and next-generation sequencing analysis. At present, the recommended test is next-generation sequencing, including whole-genome sequencing, Guide-seq, Digenome-seq. And next-generation sequencing is now widely applied to predict and detect off-target mutations in CRISPR/Cas9. Each sequencing analysis has its own characteristics and is suitable for different situations, such as Guide-seq and Digenome-seq are preferred for sgRNA evaluation, while whole genome sequencing is always used to measure genetically edited cells or animals.
CIRCLE-Seq, as one of the most popular methods, being widely utilized in off-target analysis in organisms lacking complete genomic sequences. Compared to Digenome-seq, CIRCLE-seq showed a lower background by enrichment of nuclease-cleaved genomic DNA prior to sequencing. The CIRCLE-Seq service offered by Creative Biogene CRISPR/Cas9 Platform covers everything from sample preparation to sequencing to data analysis to detect off-target cuts of CRISPR/Cas9.
Besides, GUIDE-Seq is useful for assessing the genome-wide specificity of nucleases, including dimeric ZFN, TALEN and CRISPR RNA-directed nucleases as it can identify whole-target off-target sites, ranging from exons, introns, to intergenic regions. Creative Biogene CRISPR/Cas9 platform provides GUIDE-Seq service to evaluate off-target effects of CRISPR/Cas9. All projects are performed by skilled staff under the guidance of experienced scientists.
‘Our CRISPR/Cas9 Platform provides complete GUIDE-Seq services including sample preparation, sequencing and data analysis. Upon completion of the project, we will provide clients with a detailed report containing the experimental results and raw data. Moreover we can also customize the specific program for your projects based on your need.’ said Macia Brady, she also claimed, ‘Except for CIRCLE-Seq and GUIDE-Seq service, there are several other services can be applied for the analysis of off-target Testing, we are always committed to accelerating the development of gene editing analysis.’
About CRISPR/Cas9 Platform
CRISPR/Cas9 platform, as a professional division of Creative Biogene, is dedicated to supporting our clients conduct genetic research with comprehensive gene editing services and products as well as experienced in-house experts. With years of efforts. Creative Biogene CRISPR/Cas9 platform has become a world-recognized industry platform that supports millions of scientists worldwide.
Contact Info:
Name: Macia Brady
Email: Send Email
Organization: Creative Biogene
Address: 45-1 Ramsey Road, Shirley, NY 11967, USA
Phone: 6316197922
Website: https://www.creative-biogene.com/crispr-cas9/
Source: MarketersMedia
Release ID: 88899310
